Description: Adenovirus is so far the most efficient gene delivery vector with nearly 100% efficiency for both dividing and non-dividing mammalian cells while AAV and lentivirus usually give 30~40% infection efficiency. The custom shRNA adenovirus service is to convert the unsurpassable efficiency of adenovirus to effective gene silencing on all mammalian cells for both in vitro or in vivo applications. Our proprietary Ad.MAX? technology provides maximum shRNA adenovirus production and more flexibility to meet customers' needs. Ready-to-use deliverables are suitable for in vitro and in vivo applications.
If commercial shRNAs or siRNAs of your target gene are not available or non-functional, or your project requires special design for further development, we offer special shRNA design without additional charge!
Advantages: - One-stop service: from shRNA design to packaging. - Unsurpassable high infection efficiency. - Quick turnaround time: 3 to 4 weeks. - Cost-effective. - Special design request acceptable, FREE!
Wide choice of Ad.MAX? shuttle vectors for shRNA cloning: Requirements: GenBank access #, sequence of gene of interest or shRNA is needed.
Deliverables: Total 2.0 ml at 2x1010~5x1011 PFU/ml shRNA adenovirus stock will be delivered.
Options: - Wide choice of selection markers. - Constitutive or inducible. - Concentration for in vivo application. - Packaging-only if you already have a construct. - Re-order of additional volume at reduced cost. - Control virus: vector-only or scrambled shRNA. The most popular full and customized shRNA adenovirus construction services with Ad.MAX? system are being listed below. We are offering discount for new customers. Please inquire with us for pricing for your special needs.
Recombinant Type 5 shRNA Adenovirus (E1/E3 deletion) with Ad.MAX System:
– One-stop service: from shRNA design to packaging.
– Unsurpassable high infection efficiency.
– Cost-effective.
– Special design request acceptable, FREE!
Wide choice of Ad.MAX? shuttle vectors for shRNA cloning:
Service Description:
1. Synthesize and clone shRNA into adenovirus vector.
2. Viral production in Ad.MAX? 293 cells: transfection and purification of recombinant adenovirus.
3. Gener...
Recombinant Type 5 shRNA Adenovirus (E1 and E3 deletion) Amplification with Ad.MAX System:
– Exceptional gene delivery efficiency (nearly 100%) in most cell types including dividing and non-dividing or primary cells.
– No integration into host genome.
– Induce efficient gene silencing.
– Wide choice of Ad.MAX? shuttle vectors.
Service Description:
1. Generation of high titer recombinant adenoviral stocks from ~1000 cm2 of Ad.MAX? 293 cells.
2. Adenovirus PFU titrat...
Recombinant Type 5 shRNA Adenovirus (E1 and E3 deletion) Amplification with Ad.MAX System:
– Exceptional gene delivery efficiency (nearly 100%) in most cell types including dividing and non-dividing or primary cells.
– No integration into host genome.
– Induce efficient gene silencing.
– Wide choice of Ad.MAX? shuttle vectors.
Service Description:
1. Generation of high titer recombinant adenoviral stocks in ~4000 cm2 of Ad.MAX? 293 cells.
2. 2xCsCl ultracentrifuge ...
Cell-based shRNA Validation Service:
– Cloning cDNA of your targeted gene into a validation plasmid
– Design and synthesize ~6 shRNA oligoes followed by cloning them to a shuttle vector.
– Transient co-transfection cDNA/shRNA plasmids.
– Quantification of target gene silencing via qRT-PCR
Available shRNA Validation Platforms:
– Constitutive shRNA knockdown
– Inducible shRNA knockdown
Guaranteed Knockdown:
We guarantee the knockdown of >80% at mRNA level...
