GenJet? In Vitro DNA Tranfection Reagent for Neuro-2A Cells
￥ 1,560.00
購買(mǎi)數量：
目錄號: SL100489-N2A
包裝: 1.0 ml
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Description
GenJet? DNA In Vitro Tranfection Reagent for Neuro2A is pre-optimized for transfecting Neuro2A cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on Neuro2A cells. GenJet? reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 100 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
90%
Cell culture conditions
DMEM with 4.5 g/L glucose, 10% FBS
GenJet? (μl) : DNA (μg) Ratio
3:1
Diluent for DNA and Transfection Reagent
Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex
15 minutes at RT
Presence of Serum/Antibiotics during Transfection
Yes
Change Medium 5 Hours After Transfection
Yes
Maximal Efficiency
48 hours
Transfection Results:
Reporter Gene
EGFP
Plasmid
pEGFP-N3 (CMV promoter)
Efficiency (GFP %)
89%
Storage Condition Store at 4 °C. If stored properly, the product is stable for 12 months or longer A Picture Showing Transfection Efficiency of GenJet? Reagent on Neuro2A Cells.? Neuro2A cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet? and visualized by a Nikon Eclipse Fluorescence microscope with DIC phase (left panel) and FITC (right panel) imaging 36 hours post transfection
