亚洲第一无码XXXXXX,免费无人区一码二码乱码,成全视频在线观看高清动漫,永久免费的啪啪免费网址


GenJet? In Vitro DNA Tranfection Reagent for Ca Ski Cells

￥ 1,560.00

購買(mǎi)數量：

  • 目錄號: SL100489-CaS
  • 包裝: 1.0 ml

請選擇:



Description

GenJet? DNA In Vitro Tranfection Reagent for Ca Ski Cell is pre-optimized for transfecting Ca Ski Cells, human cervix epidermoid carcinoma cells.? Refer to the following optimal transfection conditions for maximal transfection efficiency on Ca Ski cells. GenJet? reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Confluence on the day of transfection
 80%
Cell culture conditions DMEM with 4.5 g/L glucose, 10% FBS
GenJet? (μl) : DNA (μg) Ratio 3:1
Diluent for DNA and Transfection Reagent
 Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex 15 minutes at RT
Presence of Serum/Antibiotics during Transfection Yes
Change Medium After Transfection Yes, 18~24 Hours After Transfection
Maximal Efficiency 48 hours

Transfection Results:

Reporter Gene
 EGFP
Plasmid
 pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 88%

Storage Condition
 Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet




版權所有 © 2024 思科生物科技有限公司.