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GenJet? In Vitro DNA Tranfection Reagent for SK-OV-3 Cells

￥ 1,560.00

購買(mǎi)數量：

  • 目錄號: SL100489-SKOV3
  • 包裝: 1.0 ml

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Description

GenJet? DNA In Vitro Tranfection Reagent for SKOV3 cell is pre-optimized for transfecting SKOV3 cells.

Refer to the following optimal transfection conditions for maximal transfection efficiency on SKOV3 cells. GenJet? reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Confluence on the day of transfection
 90%
Cell culture conditions DMEM with 4.5 g/L glucose, 10% FBS
GenJet? (μl) : DNA (μg) Ratio 3:1
Diluent for DNA and Transfection Reagent
 Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex 15 minutes at RT
Presence of Serum/Antibiotics during Transfection Yes
Change Medium After Transfection Yes, 18~24 Hours After Transfection
Maximal Efficiency 48 hours

Transfection Results:

Reporter Gene
 EGFP
Plasmid
 pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 82%

Storage Condition
 Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet




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