GenJet? In Vitro DNA Tranfection Reagent for BHK-21 Cells
￥ 1,560.00
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目錄號: SL100489-BHK
包裝: 1.0 ml
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Description
GenJet? DNA In Vitro Tranfection Reagent for BHK-21 cell is pre-optimized for transfecting BHK-21 cells. BHK-21 cells is one of the most commonly used cells for vaccine production. When used for this application, usually a cell strain adapted to grow in suspension is used.
Refer to the following optimal transfection conditions for maximal transfection efficiency on BHK-21 cells. GenJet? reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
80%
Cell culture conditions
DMEM with 4.5 g/L glucose, 10% FBS
GenJet? (μl) : DNA (μg) Ratio
3:1
Diluent for DNA and Transfection Reagent
Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex
15 minutes at RT
Presence of Serum/Antibiotics during Transfection
Yes
Change Medium After Transfection
Yes, 5 Hours After Transfection
Maximal Efficiency
48 hours
Transfection Results:
Reporter Gene
EGFP
Plasmid
pEGFP-N3 (CMV promoter)
Efficiency (GFP %)
85%
Storage Condition Store at 4 °C. If stored properly, the product is stable for 12 months or longer Data Sheet
