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GenJet? In Vitro DNA Tranfection Kit for Keratinocytes

￥ 1,680.00

購買(mǎi)數量：

  • 目錄號: SL100489-KERA
  • 包裝: 1 kit (2x1.0 ml)

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Contents Per Kit:
 ● 1×1.0 ml of GenJet? DNA Transfection Reagent
● 1×1.0 ml (x50) of HapiCell? Transfection Toxicity Removal Cocktail

Description

GenJet? DNA In Vitro Tranfection Reagent for keratinocytes is pre-optimized for transfecting human or mouse primary or immortalized keratinocytes. Keratinocytes are hard to transfect partly due to the transfection toxicity related cell death. In combination of our proprietary HapiCell? Toxicity Removal Cocktail, the pre-optimized transfection reagent gave up to 50% efficiency with minimal cell death.

Refer to the following optimal transfection conditions for maximal transfection efficiency on keratinocytes. GenJet? reagent, 1.0 ml, is sufficient for ~600 transfections in 24 well plates or ~300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:

Confluence on the day of transfection
 90%
Cell culture conditions DMEM with 4.5 g/L glucose, 10% FBS
GenJet? (μl) : DNA (μg) Ratio 3:1
Diluent for DNA and Transfection Reagent
 Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex 15 minutes at RT
Presence of Serum/Antibiotics during Transfection Yes
Change Medium After Transfection Yes, ~5 Hours After Transfection
Treatment with HapiCell? Toxicity Removal Cocktail Yes, ~5 Hours After Transfection Followed by PBS Wash
Maximal Efficiency 48 hours

Transfection Results:

Reporter Gene
 EGFP
Plasmid
 pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 56%

Storage Condition
 Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet




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