GenJet? In Vitro DNA Tranfection Reagent for 3T3 Cells
￥ 1,560.00
購買(mǎi)數量：
目錄號: SL100489-3T3
包裝: 1.0 ml
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Description
GenJet? DNA In Vitro
Tranfection Reagent for NIH3T3 Cells is pre-optimized
for NIH3T3 cell transfection.
NIH 3T3 cells come from a cell line established in 1962 by two scientists then
at the Department of Pathology in the New York University School of Medicine,
George Todaro and Howard Green. The 3T3 cell line has become the standard
fibroblast cell line. Todaro and Green originally obtained their 3T3 cells from
Swiss mouse embryo tissue.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on NIH 3T3 cells. GenJet? reagent, 1.0 ml, is sufficient for 300 to
600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
~70%
Cell culture conditions
DMEM with 4.5 g/L glucose, 10% FBS
GenJet? (μl) : DNA (μg) Ratio
3:1
Diluent for DNA and Transfection Reagent
Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex
15 minutes at RT
Presence of Serum/Antibiotics during Transfection
Yes
Change Medium 5 Hours After Transfection
No
Maximal Efficiency
48 hours
Transfection Results:
Reporter Gene
EGFP
Plasmid
pEGFP-N3 (CMV promoter)
Efficiency (GFP %)
90%
Storage Condition Store at 4 °C. If stored properly, the product is stable for 12 months or longer A Picture Showing Transfection Efficiency of GenJet? Reagent on NIH 3T3 Cells. NIH 3T3 cells
were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by
GenJet?. The efficiency was checked 24 hours post transfection Data Sheet To request a free trial sample, please Create An Account with us to enter your shipping address and email us at order@signagen.com
